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rpmEB [2019-01-30 13:50:57]
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rpmEB [2019-01-30 13:50:57]

general stress protein, binds in the stationary phase to the ribosome, replaces RpmE under conditions of zinc limitation
locus
BSU30700
pI
9.81
mw
9.39 kDa
protein length
82 aa Sequence Blast
gene length
249 bp Sequence Blast
function
survival of salt stress
product
accessory ribosomal protein
essential
no
synonyms
rpmE2,ytiA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    3,138,978 → 3,139,226

    The protein

    Protein family

  • Type B subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|035154A4160F084BA1CC124D0951937B203E167D|RpmE]

    • Modification

  • phosphorylated on Arg-70 [Pubmed|22517742]

    • Structure

  • [PDB|5O5J] (30S ribosome subunit of Mycobacterium smegmatis, 43% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [PubMed|12904577,15049826], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]
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    Biological materials

    Mutant

  • MGNA-A292 (ytiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/292 NBRP B. subtilis, Japan]
  • BKE30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT, downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT
  • BKK30700 (Δ[gene|DAE7AB27A4C42BF50556BB76F33BEFA8DF35EA3A|rpmEB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCTCCTTTCAAT, downstream forward: _UP4_TAAGGCAGGCCTGAGGGTTT

    • References

  • 17163968,15049826,16547061,12904577,22383849,15805528,19648245,22517742,23002217,27561249